P. J. Šafárik University in Košice >> Faculty of Science >> Institute of Chemistry

Department of Biochemistry

 Laboratory of Biochemistry and Strucure Analysis of Biomacromolecules

Subjects of the Study


Biochemistry I

Teachers: Antalík

Annotation: The lectures will give a survey of new technologies and materials utilized as alternative replacements of human tissues and parts of human organs. Examples of practical exploitation of synthetic and recombinant biomaterials will be given. The lectures are suitable for both scientific- and pedagogically oriented students.


Biochemistry II

Teachers: Antalík

Annotation: Structures and functions of saccharides and lipids. Metabolism, regulation of metabolic pathways. Basic metabolic processes - oxidative phosphorylation, glycolysis, phentose phosphate pathway, citric acid cycle, gluconeogenesis, oxidation of fatty acids, amino acids degradation and the urea cycle, and photosynthesis. Principle of bioenergetic. Hormones and vitamines.


Biochemistry - for biology and interdisciplinary studies

Vyučujúci: Tomášková

Anotácia:Protein Structure and Function, Exploring proteins, DNA and RNA and the Flow of Genetic Information, Exploring genes, Enzymes: Basic Concepts and Kinetics, Catalytic Strategies and Regulatory Strategies, Carbohydrates (Monosaccharides, Disaccharides, Polysaccharides – Functions and Properties), Lipids and Cells Membranes, Membrane Channels and Pumps, Metabolis: Basic Concepts and Design, Signal-Transduction Pathways, Glycolysis and Gluconeogenesis, Glycogen Metabolism, The Citric Acid Cycle and Glyoxylate Cycle, Oxidative Phosphorylation, The Light Reactions of Photosyntesis, The Calvine Cycle and the Pentose Phosphate Pathway, Fatty Acids Metabolism, Urea Cycle, DNA Replication, Transcription (RNA Synthesis), Protein Synthesis & Degradation, the Integration of Metabolism


Modern trends in biochemistry

Teachers: Víglaský

Annotation: Biochemical view into the modern trends: evolution of energetic metabolism, protein splicing, protein sequencing, protein - DNA interaction, problems of DNA replication, cell division, viruses, apoptosis.


Practices of biochemistry

Trainers: Kožurková, Sabolová, Varhač, Tomášková

Annotation: Important biochemical laboratory methods. Quantitative methods assignments of amino acids and proteins. Isolation casein from milk and assignment of izoelectric point of casein. Isolation of gluthation from yeast. Assignment of proteins concentration by Lowry method and dialysis of proteins. Reactions for identification of sugars. Extraction of fat from anise seeds by Soxlet apparatus. Fats constants: define of iod number by Yasud method and assignment of number of saponify. Reactions for demonstration of cytochrome oxidase, suctinat dehydrogenase and xantin dehydrogenase. The substrate specifity for glycosidases: sacharases and alpha-amylases, pH influence on alpha-amylase activity. Influence concentration of substrate on initial rate of reaction: assignment of Km and Vmax for urease. Isolation of nucleic acids: DNA for spleen and RNA from yeast.


Bioenergetic and bioelectronics

Teachers: Antalík

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Proteins - structure and function

Teachers: Varhač

Anotácia: Characterization of main principles in biosynthesis and structure of proteins. Analysis of the structure-function relation in proteins. Methods utilized for protein studies.


Enzymology

Teachers: Antalík

Anotácia: To learn to use the basic equations of enzyme kinetics. Ability to determine basic kinetic and thermodynamic parameters of enzyme catalyzed reaction.


Biophysical chemistry I

Teachers: Antalík

Annotation: The basic principles of structure, dynamics and stability of biological systems. Principles of evolution and transition from chemical to biological form of matter activity. Coherence between micro- amd macro-quantities describing biologica systems.


Biophysical chemistry II

Teachers: Antalík

Annotation: Introduction into methods of physical chemistry utilized for the study of biological systems.


Biophysical chemistry - Practices

Teachers: Antalík

Annotation: The use of physical chemistry to measure and understand the properties of biomacromolecules.


Biomaterials

Teachers: Antalík

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Biotechnology

Teachers: Sabolová

Annotation: The application of basic biotechnological processes in the chemical industry and agriculture, preparation of organic acids, amino acids, vitamines, ethanol, beer, wine, yeast etc. The liquidation of scraps by using a biotechnological processes.


Practices of biotechnology

Teachers: Sabolová

Annotation: The application of basic biotechnological processes in the chemical industry and agriculture, preparation of organic acids, amino acids, vitamines, ethanol, beer, wine, yeast etc. The liquidation of scraps by using a biotechnological processes.


Molecular biology I

Teachers: Víglaský

Annotation: Metabolism of the nucleic acids, control and regulation of the gene-expesion, storage of genetic information. The genome of prokaryotic and eukaryotic cells. The cell differentiations on the base of molecular level. Molecular mechanism of the transcription, translation and replication.


Molecular biology II

Teachers: Víglaský

Annotation: Classification of the viruses, the effect of physical and chemical factors to the viruses. Biochemistry of the viruses, viral replication, viral oncogenicity, retroviruses and HIV. DNA mutation and reparation mechanism. Oncogenes, neoplastic transformation, molecular fundamentals of cancerogenesis.


Practices of molecular biology

Teachers: Víglaský

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PC analysis of biomacromolecules

Teachers: Pristaš

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Biochemistry of microorganisms

Teachers: Kožurková

Annotation: Alternative metabolic pathways of microorganisms, production of secondary metabolites, exploitation of alternative metabolic pathways for synthesis of precursors xenobiotic.


Biochemistry of physiological processes

Teachers: Tomášková

Annotation: Biological membranes, membrane transport, transport of water and organic molecules, ion transport, transport of macromolecules, erythrocytes and transport of gases. Structure of hemoglobin and myoglobin, energetic metabolism: integration and organ specialization, metabolic function of liver, metabolic function of saddles, function of pancreas and his endocrine activities, nerve cell, briefing of structure of spinal cord and brain, nerve fiber, distribution of nerves, functional organization of nerve system, autonomous nerve system, physiology of muscle contraction.


Biochemical analytical methods

Teachers: Varhač

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Xenobiochemistry

Teachers: Sabolová

Annotation: Basic characterization and partition of xenobiotics. Metabolism of the foreing matter in the liver. The nature of biotransformation reactions, their induction and inhibition. Biochemical tests and their applying in the environment analysis.


Seminar of thesis

Teachers: Kožurková

Annotation: Seminary preparation of the students to writting thesis.


Clinical biochemistry

Teachers: Molčányiová

Annotation: Biological molecules: lipides, carbohydrates, proteins, metabolism of biological molecules. Liver, kidneys, GIT and the circulatory system: properties and functions. Endocrine system: hypothalamus, pituitary, thyroid hormones, sex hormones.


Patobiochemistry

Teachers: Molčányiová

Annotation: Carbohydrate metabolism in relation to liver physiology and disease, lipid metabolism in relation to liver physiology and disease, hypercholesterolemia and effects of hormones, protein degradation and clinical aspects, alcohol and the liver, disease of kidneys, hepatitis, heart and circularly systems disease.



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